Mammalian Cell Culture Protein Expression

Mammalian cell expression has become the dominant recombinant protein production system for clinical applications because of its capacity for post translational modification and human protein like molecular structure assembly.

Mammalian cell culture protein expression.

Cho cells can grow very well in serum free cell culture medium which eliminates serum as a potential source for infectious agents providing more safety. This is where mammalian cells can really shine creating more native natural proteins and effective antibodies. Widely used for the production of recombinant proteins for structural studies insect expression platforms are inexpensive scalable and less demanding than mammalian cells. Chinese hamster ovary cho cells which have been successfully used for the manufacture of fda approved therapeutic proteins and antibodies are prominent in stable expression stably transfected cho cell line is the most suitable mammalian host for high yield production of recombinant proteins.

The gibco expi293 membranepro expression system combines the benefits of the expi293 expression system and the gibco membranepro expression system. In last two decades mammalian cell protein expression has become the dominant recombinant protein production system for clinical applications producing more than half of the biopharmaceutical products on the market and several hundreds of candidates in clinical development. Cho cells are excellent in protein folding of complex proteins including their posttranslational modification ptm. Therefore the mammalian expression system is suitable for expressing complicated modified functional proteins antibodies.

Secreted or membrane proteins such as recombinant glycoproteins vaccines and enzymes. Cho cells grow mostly in suspension allowing volumetric. It is designed for high yield transient expression and display of mammalian cell surface membrane proteins including g protein coupled receptors gpcrs in an aqueous soluble format. Coli make excellent organisms for expression of several types of proteins but do have limitations based on their cellular hardware.

This blog focuses on the differences between bacterial and mammalian cell expression systems. While expression and production have been fully developed and chinese ham. These cells can be grown in spinner or shaker flasks and do not require a co 2 enriched atmosphere established insect cell lines may produce recombinant proteins with high yields and require smaller culture. Why cho cells are frequently used for mammalian protein expression.